simple technique for three-dimensional imaging and segmentation of brain vasculature u sing fast free-of-acrylamide clearing tissue in murine

ABSTRACT

Objective: Fast Free-of-Acrylamide Clearing Tissue [FACT] is a recently developed protocol for the whole tissue three-dimensional [3D] imaging. The FACT protocol clears lipids using sodium dodecyl sulfate [SDS] to increase the penetration of light and reflection of fluorescent signals from the depth of cleared tissue. The aim of the present study was using FACT protocol in combination with imaging of auto-fluorescency of red blood cells in vessels to image the vasculature of a translucent mouse tissues