Antioxidant activity, Hypoglycemic potential and metabolite profiling of Hyophorbe indica leaf extract

ABSTRACT

Current work was performed to evaluate antioxidant activity, ?-glucosidase inhibition, phytochemical profiling and in vivo hypoglycemic activity of freeze dried, ultrasonicated Hyophorbe indica leaf extracts. The highest total phenolic contents of 208.77 +/- 2.11 mg GAE/g DE and total flavonoid contents of 173.90 +/- 2.30 mg Rutin/g DE were obtained in 60% ethanol extract. The 60% ethanol extract exhibited maximum DPPH radical scavenging with IC50 value of 35.35 +/- 0.189microg/mL and total antioxidant power of 330.26 +/- 3.13 [ASE/g PE], respectively. The highest ?-glucosidase inhibition [IC50 36.52 +/- 0.08?g/mL] was also observed for 60% ethanol extract. The 60% ethanol extract at dose of 450 mg/kg body weight reduced blood glucose level of alloxan induced diabetic mice by 51.41% which was quite comparable with metformin [56.67%]. Twelve compounds namely citric acid, procyanidin B3, epicatechin, procyanidin B2, catechin, catechin derivative, procyanidin B1, apigenin-c-hexocide-c-hexocide, kaempferol, kaempferol derivative, quinic acid derivative and gallic acid have been identified by using UHPLC-Q-TOF-MS/MS in 60% ethanol extract