correlation study between 2,3-diphosphoglycerate [2,3-DPG] and platelet aggregation on congenital heart diseases

ABSTRACT

This study was conducted upon 30 individuals, 20 patients having congenital heart diseases [CHD], 10 cyanotic and 10 acyanotic patients, and 10 healthy controls. All subjects were investigated for 2, 3-diphosphoglycerate [2, 3-DPG] in blood and ADP induced platelet aggregation to clarify any relationship between these two parameters. Also platelet aggregation to clarify any relationship between these two parameters. Also platelets count, hematocrit value, [HCT], O2 saturation, PO2 and blood pH were determined. The results revealed that platelet did not differ in patient groups compared to control group. In the cyanotic group ADP-induced platelet aggregation showed a marked impairment compared to control group. In addition 2, 3-DPG enzyme was significantly increased in both cyanotic and acyanotic groups, but this increase was much higher in the cyanotic group. Correlation study between 2, 3-DPG level in blood and platelet aggregation induced by revealed that there was an inverse relationship between 2, 3-DPG enzyme level in blood and ADP induced platelet aggregation in patients with cyanotic CHD. Thus, it could be concluded that the glycolytic pathway of red blood cells interact with the prostaglandin pathway of platelets in order to maintain blood flow homeostasis in hypoxic patients. If this is proved to be correct, then, there is another good reason apart from altering hemoglobin oxygen affinity, for elevated 2, 3-DPG concentration in erythrocytes in cyanotic patients. Such an enzyme could be of great clinical usefulness in the treatment of thromboembolic disease