Effect of potassium cyanide [KCN] and sodium azide NaN3 on the growth of streptococcus pyogenes Gr A

ABSTRACT

In order to show that the growth of S. pyogenes Group A was independent of both Fe and O2 as a terminal electron acceptor, KCN and NaN3 were added to the growth medium at levels which completely inhibited growth of aerobic bacteria. These agents have no effect on the growth of S. pyogenes at concentration below 100 mM, proving that the growth was independent of cyt aa3 oxidoreductase and O2