Some properties of crude phospholipase C enzyme from Oxystelma Esculantum

ABSTRACT

Phospholipase C activity was demonstrated from axystelma esculantum root tuber. The optimum enzyme activity was found at pH 5.5 and temperature 30°C. Enzyme activity was stimulated with Triton x - 100 [1mM] 25%, sodium deoxycholate [1mM] 65%, cobalt chloride [5mM] 162.5% and Zinc chloride [10mM] 180%. Enzyme activity showed 100% inhibition with EDTA [1mM] and Calcium chloride [10mM]. Oxystelma esculantum crude Phospholipase C enzyme activity was found to be heat labile