DNA-feulgen cytophotometry in hyperplasia and adenocarcinoma of the endometrium

ABSTRACT

The present study was carried out on 35 endometrial hyperplasia, 30 endometrial carcinoma and 5 normal proliferative phase endometrium. Cases were stained with hematoxylin and eosin and Feulgen stain for cytophotometry. The majority of cells in the normal and the hyperplastic groups, including atypical hyperplasia were within the diploid range, whereas more than 65% of the cells in the carcinoma group, were aneuploid with increase in number of aneuploid cells as the grade of the tumor increased. The number of aneuploid cells was also proportionate to the nuclear area measurements. Small nuclear areas were usually diploid, while larger ones were associated with more DNA and were aneuploid content. Such results indicated that cytophotometry may be a helpful adjuvant in routine histolopathological diagnosis, increasing the diagnostic accuracy and shedding light on the biological potential of the malignant cells