Immunophenotypic analysis of non-Hodgkin's lymphoma

One hundred and three cases of Non-Hodgkin's lymphoma were evaluated immunohistochemically using a panel of monoclonal antibodies which includes leucocyte common antigen [LCA], CD45R [Pan-B marker], L-26 [CD 20-Pan-B marker] and UCHL-1 [Pan-T marker]. Of the total 63 cases [61.17%] showed a B-cell phenotype while 40 [38.83%] were of T-cell origin. Most B-cell neoplasms belonged to intermediate [79.36%] or high grade [15.87%] according to the International Working Formulation [WF]. Most T-cell lymphomas were of either intermediate [52.5%] or high grade [32.5%] neoplasms. Some T-cell neoplasms presented as specific clinicopathological entities like lymphomatoid granulomatosis [2 cases], mycosis fungoides [1 case] and AILD type NHL [1 case]. In 27 cases the immunostaining pattern of two. Pan-B markers i.e., L26 and CD45R was compared. L26 staining was expressed in all 27 cases [100% sensitivity] while CD45R showed positive reaction in 22 cases [82% sensitivity]. UCHL-1 is proved to be a sensitive and lineage specific T-cell marker and in 67% cases the staining pattern was moderate [+2] to intense [+3]. The mean age for the B-cell lymphomas was 49 years and 36 years in T-cell neoplasm. Male to female ratio in both types of lymphomas was 2:1. The study indicates a high prevalence of T-cell when comparing the data from western countries and lower to those from Japan and Caribbean countries