Enzyme linked immunosorbent assay for dexamethasone in equine urine
PJMR-Pakistan Journal of Medical Research. 1997; 36 (3): 75-79
Dans Anglais
| IMEMR
| ID: emr-46559
ABSTRACT
A simple and sensitive competitive Enzyme Linked Immunosorbent Assay [ELISA] with a colorimetric end point has been developed for the measurement of dexamethasone in equine sample. Use of pre-mixed reagent of untreated sheep antiserum and donkey antisheep alkaline phoshatase-labelled second antibody enables analysis of 40 duplicate urine sample within 75 minutes. The assay has good precision [Sr less than 5% for within and between assay runs] and is sensitive [LOD equivalent to 173 ng ml-l of diluted equine sample]. The assay has been used to detect the drug in urine over 20 hours in urine from a race horse following intramuscular [i.m.] injection of 20 mg of dexamethasone sodium phosphate
Recherche sur Google
Indice:
Méditerranée orientale
Sujet Principal:
Dexaméthasone
/
Test ELISA
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Pak. J. Med. Res.
Année:
1997
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS