Human cystic echinococcosis: diagnostic value of different antigenic fractions of hydatid cyst fluid with different specific immunoglobulin g subclasses by enzyme linked immunoelectrotransfer blot

ABSTRACT

The present study aimed to evaluate the usefulness of immunoblot analysis of hydatid cyst fluid [HCF] for diagnostic verification of human cystic echinococcosis [CE]. HCF of camel origin was resolved by SDS-PAGE gel electrophoresis and transblotted on nitrocellulose membrane. Forty-four persons with other parasitic infections and 20 normal controls were analyzed. Total IgG and IgG subclass 1-4 in CE sera preferentially recognized several polypeptide bands in the range of 14-200 kDa. Sera from the normal controls did not recognize any of these polypeptides. These data suggested that detection of any of these polypeptides bands could be used for the confirmation of human cystic echinococcosis in Egypt