Isolation and structural characterization of toxocara vitulorum specific antigen and its potency in diagnosis of toxocariasis among buffalo calves
Journal of the Egyptian Society of Parasitology. 2000; 30 (2): 387-400
Dans Anglais
| IMEMR
| ID: emr-54162
ABSTRACT
A method of affinity chromatography purification of species-specific antigen from Toxocara vitulorum adult worm was described. The purification process resulted in a fraction with 9315 fold increased in specific activity compared to crude extract. Structural characterization of the isolated fraction by SDS polyacrylamide gel electrophoresis, isoelectric focusing and amino acid analysis showed that the fraction consists of only two polypeptides of 92 kD and 87 kD with isoeletric points of 8.8 and 8.9. Moreover, 17 amino acids were identified in the fraction with high proportions of only three of them [tyrosine, glycine and glutamic]. The isolated antigen proved potency in the diagnosis of toxocariasis among buffalo claves using ELISA which recorded 100% sensitivity
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Indice:
Méditerranée orientale
Sujet Principal:
Toxocara
/
Buffles
/
Test ELISA
/
Toxocarose
/
Techniques et procédures diagnostiques
/
Électrophorèse sur gel de polyacrylamide
/
Animaux domestiques
/
Antigènes d'helminthe
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
J. Egypt. Soc. Parasitol.
Année:
2000
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