Diagnosis and differentiation between E. histolytica and E. dispar by PCR technique

ABSTRACT

Amoebiasis caused by the enteric protozoan E. histolytica, is one of the major parasitic diseases in the world, it is responsible for up to 100,000 deaths per year. It is common in developing countries with poor socioeconomic conditions and malnutrition. It is well established recently the clear distinction of two separate species which are morphologically indistinguishable E. histolytica and E. dispar the later species is proved to be non- pathogenic and does not require any treatment. This work was done to evaluate PCR technique as a method for diagnosis and discrimination between the two species, and to assess the incidince of this parasite in Qualiubia Province. The study included 200 cases from out patient clinic. History front the patient was taken, the samples were subjected to macroscopical and microscopical stool examination, formol-ether concentration of samples, and PCR of the DNA extracted from stool samples. All the results obtained were analysed statistically