Expression of alkaline phosphatase during ostesgenic differentiation of rat bone marrow stromal cells

ABSTRACT

Bone marrow contains a population of stem cells capable of differentiating to osteoblast and forming the bone nodule by dexamethasone. The stromal cells of bone marrow obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for 7 days and subcultured for 18 days. The cells were cultured in either DMEM medium containing 15 percent fetal calf serum and antibiotics as the controls or the above medium supplemented with Osteogenic supplements [OS] include 10 mM Na-Beta glycerophosphate [Na-Beta Gp][5] 10 nM dexamethasone [Dex] and 50 g/ml ascordic acid [AsA] as the examined cultures. After 6, 12 and 18 days of grow up in subculture, the cultures were examined for mineralization and alkaline phosphatase [Apase] expression. Mesenchymal stem cells [MSCs] in examined cultures underwent a dramatic change in cellular morphology and a significant increase in Apase activity by day 12. The deposition of a calcified matrix on the surface of the culture flasks became evident between days 12 and 18. The addition of osteogenic supplements [OS] to MSCs cultures induced Apase expression that contributes to cellular differentiation and mineralization of extracellular matrix