Genotoxic effect of diethylaminoethanol in mouse bone-marrow and germ cells
Bulletin of the National Research Centre. 2002; 27 (1): 73-84
Dans Anglais
| IMEMR
| ID: emr-59074
ABSTRACT
The genotoxic potential of the aliphatic amine diethylaminoethanol [DEAE] wasevaluated in vivo using two cytogenetic parameters, chromosomal aberrations inboth somatic and germ cells and sister chromatid exchanges [SCEs]. Theprotective effect of Royal Jelly was also tested. Male Swiss mice weretreated by gavage with DEAE at the dose levels of 100, 150 and 200 mg/kg b.wt. The used doses induced a significant percentage of chromosomalaberrations at 24 hours post-treatment, even after excluding the number ofmetaphases with gaps compared with control. Repeated treatment for three days withthe same dose induced a statistically higher percentage of chromosomalaberrations than single treatment. DEAE induced also a statisticallysignificant percentage of chromosomal aberrations in mouse spermatocytes atdiakinesis metaphase I after single and repeated treatments. The maximumpercentage of the induced aberrations reached 17.06 + 0.95 after repeatedtreatment with the highest tested dose for 3 consecutive days compared with4.8 +/- 0.33 for the controls. DEAE was found to potent inducer of SCEs atthe tested doses 100, 150 and 200 mg/kg b. wt. [P <0.01]. The meanfrequency reached 10.53 +/- 0.38, 12.97 +/- 0.41 and 15.79 +/- 0.52,respectively, compared with 5.54 +/- 0.48 for the controls. Treatment withRoyal Jelly at 1 mg/kg b. wt., decreased the percentage of totalchromosomal aberrations and the frequency of SCEs induced by DEAE in bonemarrow and spermatocyte cells
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Indice:
Méditerranée orientale
Sujet Principal:
Échange de chromatides soeurs
/
Moelle osseuse
/
Exposition professionnelle
/
Aberrations des chromosomes
/
Agents protecteurs
/
Analyse cytogénétique
/
Animaux de laboratoire
/
Souris
/
Tests de mutagénicité
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Bull. Natl. Res. Cent.
Année:
2002
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