Study of kinetics of circulating schistosome antigens in tissues and sera of infected mice using anti-schistosoma mansoni soluble egg antigen monoclonal antibody
Egyptian Journal of Schistosomiasis and Infectious and Endemic Diseases. 2002; 24: 1-17
Dans Anglais
| IMEMR
| ID: emr-59163
ABSTRACT
In the present study, the kinetics of uptake and deposition of Schistosoma mansoni antigens in liver, spleen, kidney and intestine of C57BL/6 mice infected with 100 Schistosoma mansoni cercariae as well as their levels in sera have been investigated during the course of infection [12 weeks]. The presence of antigen was demonstrated by indirect immunofluorescence using IgM anti-soluble egg antigen monoclonal antibody [anti-SEA MAb]. Immunofluorescence reactivity was evident in both renal and spleen tissues 3 weeks post-infection [p.i.], in Kupffer cells of liver 4 weeks p.i. and in intestinal mucosa [5 weeks p.i.]. Maximal immunofluorescence staining was reached during the patent phase [5-9 weeks p.i.]. During the chronic stage of infection [9-12 weeks pi.], diminution of immunofluorescent intensity was evident in liver tissue, while it remained constant in other studied organs. Circulating schistosome antigen [CSA] level in mice sera was determined using a sandwich ELISA with a MAb for both antigen capture and detecting antibody. CSA was demonstrated in mice sera [one week p.i.] reaching its peak at 6 weeks p.i. and remained at a detectable level until the end of the study [12th week p.i.]
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Indice:
Méditerranée orientale
Sujet Principal:
Sérologie
/
Test ELISA
/
Cinétique
/
Protéines d'oeuf
/
Souris
/
Anticorps monoclonaux
/
Antigènes d'helminthe
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Egypt. J. Schistosomiasis Infect. Endem. Dis.
Année:
2002
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