Adenosine A10 receptors activity of entrohinal cortex on amygdala bindled seigusrs in rat

ABSTRACT

The role of adenosine A1 receptors of the entorhinal cortex on amygdaloid kindled seizures was investigated. Material and Animals were kindled by daily electrical stimulation of amygdala. In the full kindled animals, N6-cyclohexyladenosine [CHA; 0.1, 1 and 10 mM], an adenosine A1 receptor agonist, and 8-cyclopenthyle 1,3-dimethylexanthine [CPT], an adenosine A1 receptor antagonist, at concentrations of 1 and 5mM were injected bilaterally into the entorhinal cortex. Animals were stimulated at 5, 15, 60 and 120 min post drug infusion and kindling parameters were measured. Results:Results showed that CHA at concentration of 10 mM reduced amygdala after-discharge duration, entrorhinal cortex after-discharge duration [E-ADD] and stage 5 seizure duration at 5, 15,60 and 120 min post drug injection. It also increased the latency of stage 4 seizure. But, no alteration was observed in seizure stage. At concentrations of 1 and 0.1 mM, CHA reduced E-ADD only at 5 and 15min post drug infusion. Bilateral injection of CPT into the entorhinalcortex did not alter seizure parameters. Intra-entorhinal cortex injection of CPT [5mM], 5 min before CHA [10mM], blocked the anticonvulsant effects of CHA. These results suggest that the entorhinal cortex has a role in seizure propagation from the amygdala and its adenosine A1 receptors activity have anticonvulsant effects on amygdala kindled seizures