study of distribution and number of nucleolar organizer region [NOR] associated proteins in gastritis and gastric carcinoma

ABSTRACT

Nucleolar organizer region associated proteins are a group ofnon histone proteins which act in r-DNA transcription and r-RNAprocessing. The amount of these proteins increase in transition from G1 toS phase in cell cycle. Hence these proteins can show the proliferativeindex of the cells. Material and For this purpose 40 patients [30 gastritis, 10 carcinoma] with average age of 45 years were chosen from pathology fileof Khatam AL Anbia of Zahedan and Shiraz Namazi Hospitals during theyears 1999-2001. Paraffin blocks were cut with 3-5 um thickness andstained with H-E and colloidal silver nitrate. The number of NOR werecounted blindly by two persons. Pearson correlation test showed significant correlation of NOR counting [P < 0.001, r = 96] by two persons. Mann-Whitney test showedsignificant difference of NOR between gastritis and carcinoma. The shapeand distribution of NOR in gastritis looked like diffuse black dots in nuclei,but in neoplastic cells the number of NOR were increased and theirdistribution differed extensively. It seems that NOR number is a reliable indicator of cellularproliferation in carcinoma of the stomach