Dot Elisa [Typhidot] in early diagnosis of typhoid fever

ABSTRACT

The study has been carried out on 334 suspected cases of Typhoid fever during the year 2002 on patients either admitted in children emergency Word-11 or seen at out patient department of Liaquat University Hospital Hyderabad - Pakistan. The purpose of study was to evaluate and compare conventional Widal test and qualitative dot enzyme immunoassay "Typhodit" test. The patients were selected below 15 year of age and were categorized in three groups. The overall sensitivity and specificity in "Typhidot" was fund to be 72.4% and 93.3% respectively, as compared to "Widal test" where sensitivity and specificity was found to be only 53.9%. The efficiency of Typhidot comes to be 74.7%. Thus it was concluded that Typhidot - Dot Elisa is a valuable diagnostic tool in early diagnosis of Typhoid fever