Hyoscyamine 6 - Beta - hydroxylase gene isolation from in vitro cultured roots of hyoscyamus niger l. and hyoscyamus tenuicaulis schonbeck - temesy
DARU-Journal of Faculty of Pharmacy Tehran University of Medical Sciences. 2003; 11 (1): 34-7
Dans Anglais
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| ID: emr-61789
ABSTRACT
The H6H gene for hyoscyamine 6b-hydroxylase, which converts hyoscyamine to scopolamine, was isolated from H. niger and H. tenuicaulis. The roots of 14 days sterile seedlings were transferred to a modified liquid B5 medium containing 1mM indolebutyric acid, and after appearance of the lateral roots, subcultured in a free hormone medium. Following a week, the total cellular RNA of the roots was extracted. cDNA of H6H gene was synthetized by RT-PCR, favored gene was amplified by the PCR
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Indice:
Méditerranée orientale
Sujet Principal:
Plantes médicinales
/
ARN
/
Extraits de plantes
/
Réaction de polymérisation en chaîne
/
ADN complémentaire
/
Racines de plante
/
Gènes
langue:
Anglais
Texte intégral:
J. Fac. Pharm. Tehran Univ. Med. Sci.
Année:
2003
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