Peptone-liver [P-L] a new culture medium for the Egyptian leishmania major

ABSTRACT

Leishmania major promastigotes isolated from an Egyptian ZCL patient was inoculated 4x106/ml in a new culture medium, peptone-liver [P-L], with a total concentration of 10% of FCS [fetal calf serum] and incubated at 25 +/- 1C. Meanwhile, the same number of promastigotes was inoculated in Schneider's Drosophila medium as a control. On the 10th day, the number of L. major promastigotes reached 29.25x106/ml. The same number was obtained with Schneider's Drosophila medium within four days. The new culture medium or P-L medium is cheap, easily prepared and gave a very good number of promastigotes as in Schneider's Drosophila medium, but in a longer time