Isolation of alpha-amylase isoenzymes in serum and saliva in diabetes mellitus

ABSTRACT

This research included a study of alpha-amylase isoenzymes in serum and saliva in diabetes mellitus, diabetic group were 100 samples and 80 samples as control group, their ages between [5-55] years. Three methods of isolation for alpha-amylase isoenzymes were studied in this research [gel filtration, anion exchange chromatography and electrophoresis]. By using gel filtration method for isolation alphaamylase isoenzymes in normal serum and saliva, the result of this isolation indicated that serum alpha-amylase [total alpha-amylase] was contained seven isoenzymes while in salivary alpha-amylase there were eight isoenzymes. Eleven isoenzymes for normal and abnormal total serum amylase and six isoenzymes for normal and abnormal salivary amylase were isolated by using anion exchange chromatography. By using polyacrylamide gel electrophoresis [PAGE], two or more bands and four bands were observed in normal serum and saliva respectively but seven or more bands and six bands were obtained from abnormal serum and saliva respectively