Reference ranges of lymphocyte subsets of healthy adults in Turkey

ABSTRACT

The aim of this study was to determine the reference ranges of lymphocyte subsets in serologically HIV-negative healthy adults in Turkey. Materials and Blood samples from 220 healthy adults, 105 female and 115 male, collected into tubes containing EDTA were investigated for lymphocyte subsets using flow cytometry. The age range was 18-80 years [44.80 +/- 16.69]. The mean percentage and absolute values of the lymphocyte subsets were as follows CD3 72.70 +/- 8.44%, 1,680 +/- 528 cells/micro l; CD4 47.37 +/- 9.10%, 1,095 +/- 391 cells/micro l; CD8 28.99 +/- 5.99%, 669 +/- 239 cells/micro l; CD19 10.96 +/- 4.44%, 254 +/- 122 cells/micro l and CD56 7.03 +/- 3.26%, 161 +/- 92 cells/micro l, respectively. The ratio of CD4/CD8 was 1.68 +/- 0.43. There was no statistically significant difference in the percentages and absolute values of lymphocyte subsets between the genders [p > 0.05]. Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adults in Turkey