Simultaneous HPLC-UV quantification of diltiazem and N-demethyldiltiazem in human plasma
Bulletin of Pharmaceutical Sciences-Assiut University. 2006; 29 (part.1): 1-8
Dans Anglais
| IMEMR
| ID: emr-76343
ABSTRACT
A simple and sensitive reversed phase high-performance liquid chromatographic method was developed for simultaneous quantification of diltiazem HCl and its major metabolite N-demethyldiltiazem in human plasma. The method involves one step solvent extraction of diltiazem, N-demethyldiltiazem and the internal standard, verapamil with n-hexane and diethyl ether [5050 v/v]. The mobile phase comprised 0.1 M ammonium dihydrogen phosphate-acetonitrile [6238 v/v] and triethylamine [0.08%] was added before the pH was adjusted to 5.9 with 85% phosphoric acid. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 238 nm. The completion time for assay was not more than 10 minutes and lower limit of quantification was 5 ng/ml. The calibration curve for diltiazem and its metabolite was linear over a concentration range of 5-200 ng/ml and average recovery was about 90%. The coefficient of variation and percent error values of the assay method within and between days were all less than 10%
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Indice:
Méditerranée orientale
Sujet Principal:
Chromatographie en phase liquide à haute performance
/
Surveillance des médicaments
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Bull. Pharm. Sci.-Assiut Univ.
Année:
2006
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