Molecular identity of the electropharetic pattern and the major cross-reacting immunogens of toxoplasma gondii [RH strain] and neospora caninum

The complex electrophoretic profile of the antigenic components of Toxoplasma gondii and Neospora caninum was studied. T. gondii [RH strain] trophozoite's antigens were resolved by gel electrophoresis under denaturating condition. Resolved polypeptides were tested by immunoblotting against hyper-immune serum prepared in Boscat rabbits. Immunoblot analysis revealed 6 reactive bands, which are 158, 111, 102, 86, 55 and 33 kDa, out of 10 antigenic bands of the parasite. Concerning N. caninum trophozoite's antigens, immunoblot analysis revealed 5 reactive bands, which are 159, 119, 106, 97 and 39 kDa, out of 8 antigenic bands of the parasite. Cross reactivity trials between the 2 parasites explore 3 cross-reactive bands, indicating high antigenic similarity in between