Molecular identity of the electropharetic pattern and the major cross-reacting immunogens of toxoplasma gondii [RH strain] and neospora caninum
Journal of the Egyptian Society of Parasitology. 2006; 36 (1): 139-148
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| IMEMR
| ID: emr-78285
Bibliothèque responsable:
EMRO
The complex electrophoretic profile of the antigenic components of Toxoplasma gondii and Neospora caninum was studied. T. gondii [RH strain] trophozoite's antigens were resolved by gel electrophoresis under denaturating condition. Resolved polypeptides were tested by immunoblotting against hyper-immune serum prepared in Boscat rabbits. Immunoblot analysis revealed 6 reactive bands, which are 158, 111, 102, 86, 55 and 33 kDa, out of 10 antigenic bands of the parasite. Concerning N. caninum trophozoite's antigens, immunoblot analysis revealed 5 reactive bands, which are 159, 119, 106, 97 and 39 kDa, out of 8 antigenic bands of the parasite. Cross reactivity trials between the 2 parasites explore 3 cross-reactive bands, indicating high antigenic similarity in between
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Indice:
IMEMR
Sujet Principal:
Protozooses animales
/
Lapins
/
Immunotransfert
/
Neospora
/
Réactions croisées
/
Électrophorèse sur gel d'agar
/
Antigènes
Limites du sujet:
Animals
langue:
En
Texte intégral:
J. Egypt. Soc. Parasitol.
Année:
2006