[ comparison of effects of toluene, especially noise and toluene along with noise per auditory brain stem response on rabbit]

ABSTRACT

An increase more than definite limit in sound pressure level is harmful for health mean while, organic solvents like toluene are used in industrial processes with noise widely. So, this study was going to investigate the relationship between toluene [1000ppm], noise and toluene along with noise [frequencies 4000 and 8000 Hz] per auditory Brain Stem response [ABR] on rabbits being exposed to these contaminants. This survey was done in experimental method on 48 three months old, male adult white New Zealand rabbits [1800 +/- 200 g body weight], in nine groups which were exposed to toluene [1000 ppm], noise [4000 Hz], 100 dB SPL, combination of toluene and noise conducted using click and tone burst stimuli in 110dB sound pressure level. Then the results of ABR test of groups exposed were analyzed by SPSS software. One-way variance [ANOVA] analysis was used to compare the groups and Turkey test was applied as a post hoc test for comparison among the groups. P values were obtained by Turkey test. t-test was conducted for comparison of ABR test results after and before exposing groups. Differences at the level of P<0.05 were considered statistically significant. The results of the study showed that Exposing rabbit's to combination of toluene and noise caused hearing impairment in all of frequencies auditory especially in frequencies 250, 500 and 1000Hz. So that, wave five in ABR test [tone burst stimuli] didn't formed in 250-1000 Hz 4.84 +/- 0.07 ms, group exposed to toluene 5.18 +/- 0.07ms, noise group [4000hz] 5.5 +/- 0.07 ms and in combination noise and toluene group [4000Hz] 5.79 +/- 0.07 ms. The mean of latency time of V wave in control was compared to that group noise group P=0.01, toluene group P=0.07 and combination noise and toluene group P=0.0001. According to the result of this study, both toluene and noise exposure caused the rabbits in hearing impairment but combination of toluene and noise exposure in frequencies of 4000Hz and 8000Hz was much worse than hearing impairment. So that, the ABR test on rabbits didn't formed V wave in low frequency