Molecular detection of apoptosis in mice treated with ketoprofen and metamizol as analgesic drugs

To assess the apoptotic effect of metamizol and ketoprofen in the livers, spleens and testes of treated mice. Apoptosis was evaluated in liver, spleen and testes of mice injected intraperitoneally [IP] with therapeutic and high doses of ketoprofen [5 and 7.5 mg/kg] and metamizol [25 and 37.5 mg/kg]. The samples were analyzed for the estimation of apoptosis at intervals of 1, 3 and 6 days of treatment using agarose gel electrophoresis technique. Ketoprofen induced apoptosis in liver, spleen and testes after 3 and 6 days of treatment, while after 1 day of treatment, ketoprofen acts as antiapoptotic agent. Also, metamizol induced little apoptosis in liver, spleen and testes after 1, 3, and 6 days. The increase of the optical density of apoptotic bands was time-and dose-dependent. Ketoprofen induced more observable apoptosis than that induced by metamizol. Ketoprofen shows fluctuated results where it acts as anti-apoptotic agent after 24 [hs] of treatment and apoptotic agent after 3 and 6 days. The work explains that receiving high doses for any period of time is toxic, receiving the therapeutic dose for long time is also toxic, but using the therapeutic dose for the recommended period is advisable