Fluid resuscitation of acute hemorrhage in uninephrectomized rabbits: effects on the early function of the remnant kidney

ABSTRACT

The aim of this study was to determine the effects of fluid resuscitation of acute hemorrhage on the early function and histopathology of the remnant kidney in uninephrectomized rabbits. Thirty-nine adult rabbits were studied in four groups. Group I [n = 8] included healthy controls; Group 2 [n = 10] healthy, bled animals; Group 3 [n = 10] uninephrectomized, non-bled animals; and Group 4 [n = 11] uninephrectomized, bled animals. In the hemorrhage groups, 8 mL kg[-1] of blood was drawn, and replaced with lactated Ringer's solution three times the volume of shed blood. Urine and blood samples were collected after 120-minutes of observation. None of the animals experienced hypotension during the study period. Serum and urinary electrolytes were similar between the Groups [p > 0.05]. Urine output was lower in Groups 3 and 4 than in Group 1 [p=0.001, both]. Urinary microalbumin, NAG, fractional sodium excretion and creatinine clearance were similar in all four Groups. Light microscopic evaluation revealed only slight enlargement of the proximal tubule lumen in the renal medulla of the rabbits that were both uninephrectomized and bled. We observed no deleterious effects of well resuscitated hemorrhage on early function and histopathology of the remnant kidney in uninephrectomized rabbits