Determination of HBV genotypes among HBs Ag positive blood donors in Tehran, Iran using PCR-RFLP
Iranian Journal of Public Health. 2009; 38 (1): 41-47
Dans Anglais
| IMEMR
| ID: emr-91465
ABSTRACT
Hepatitis B virus [HBV] is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a comparison of complete genomic sequences, HBV has been classified into eight genotypes A-H which show a geographical distribution. Some genotypes are associated with different clinical outcomes. Identification of HBV genotypes is important to begin and follow up the treatment. In this cross-sectional study, the serum samples of blood donors were collected from Tehran Blood Transfusion Centers in period during "2005-2006". Sera of 55 blood donors who were positive for hepatitis B surface antigen were selected. DNA was extracted using commercial kit and the S gene sequence was amplified by nested-PCR. PCR products were then analyzed for restriction enzymes that would be genotype specific. Genotype D was found the only type in all HBV DNA positive serum samples, in Tehran. Genotype D is dominant among Tehran's blood donors, which is consistent with Iran and the Middle East dominant genotype
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Indice:
Méditerranée orientale
Sujet Principal:
Donneurs de sang
/
Polymorphisme de restriction
/
Réaction de polymérisation en chaîne
/
Études transversales
/
Génotype
/
Antigènes de surface du virus de l'hépatite B
Type d'étude:
Étude de prévalence
Limites du sujet:
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Iran. J. Public Health
Année:
2009
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