Increased oxygen free radical production from isolated human pmnls and whole blood by luminol-enhanced chemiluminescence in autistic children
KMJ-Kuwait Medical Journal. 2009; 41 (1): 26-30
Dans Anglais
| IMEMR
| ID: emr-92029
ABSTRACT
To investigate the estimated capacity of polymorphonuclear leucocytes [PMNLs] and whole blood to produce reactive oxygen species [ROS] in children with autism and mental retardation, and compare it with normal children. Cohort study conducted between January and April 2007. Chemiluminescence laboratory [CL], Department of Physiology, Faculty of Medicine, King Khaled University Hospital, Riyadh, Saudi Arabia. Forty autistic and eight mentally retarded children. Oxygen free radical production [O[2]-, H[2]O[2], OH-] was detected by luminol-enhanced chemiluminescence, from isolated PMNLs and whole blood, stimulated by phorbol myristate acetate [PMA] and opsinized zymazan [OPZ]. Oxygen free radical production from whole blood and PMNLs. Forty autistic [35 male and fivefemale],andeightmentally retarded children [study group] were compared with forty six normal Saudi children [control group]. The mean age was 7.4 +/- 0.5 years. The CL peak response of whole blood and PMNLs stimulated with PMA and OPZ, in autistic children was significantlyhigher [p < 0.05]compared to control children. However, the CL peak response in children with mental retardation did not show any significantdifferenceswhencomparedtothecontrolgroup. There is an increase in oxygen free radicals production from whole blood and from PMNLs in autistic children. Therefore, an increase the antioxidant consumption in autistic children is strongly recommended
Recherche sur Google
Indice:
Méditerranée orientale
Sujet Principal:
Oxygène
/
Enfant
/
Études de cohortes
/
Luminescence
/
Radicaux libres
/
Mesures de luminescence
/
Luminol
/
Déficience intellectuelle
/
Granulocytes neutrophiles
Type d'étude:
Etude d'incidence
Limites du sujet:
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Kuwait Med. J.
Année:
2009
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