Effects of different doses of bone morphogenetic protein 4 on viability and proliferation rates of mouse embryonic stem cells

ABSTRACT

In this study, we examined the effect of different doses of bone morphogenetic protein 4 [BMP4] on CCE mouse embryonic stem cells [ESCs] viability and proliferation rates in order to improve the outcome of induction processes and make a system with highest viability and proliferation rates for further studies on BMP4 roles in multiple developmental stages. Expression of Oct-4 was studied and confirmed in this cell line immunocytochemically. Also, in order to evaluate the proliferation and viability rates in BMP4-treated cells, ESCs were cultured in Dulbecco's Modified Eagle Medium [DMEM] containing different doses of BMP4 [0, 0.01, 0.1, 1, 5, 25, 50 and 100ng/ml]. The mean number of whole cells and living cells were considered as proliferation and survival rates respectively. Data analysis was done with ANOVA test. The results showed that there were significant differences between the mean percent of viability between 1ng/ml and 0 ng/ml [control] and 50 and 100 ng/ml BMP4 [p mouse ESCs