[ study of the genotypic variability at the MHC [B - F] in arian broiler chickens by PCR-SSCP]

ABSTRACT

The extreme polymorphism in MHC B-F genes enables the chickens to recognize enormous numbers of foreign peptides to trigger an immune reaction. Study of these genetic resources is very important and it is essential to avoid the loss of genetic variability. Genetic polymorphism among Arian strain, a endogenous broiler chickens in Iran, was investigated. In this study PCR-SSCP was carried out for revising the polymorphism in MHC [B-F] in Arian broiler chicken. 65 samples of chicken DNA belonged to 2 groups of parents [25] and hybrids [40] were analyzed. 7 distinct profiles have been demonstrated for two groups. Two profiles were identical between two groups. Two of all profiles have been demonstrated by previous studies and 5 were new in this study. From this study it was concluded that MHC [B-F] diversity exists within Arian broiler chicken that might be useful for selecting desirable immunological traits