Effect of protamine on bacterial transformation

The effect of protamine sulphate on the genetic transformation of B. subtilis as studied and slowed that at low concentrations [10-25 micro b/ml] increased the number of the transformants to about 150%. Concentrations of protamine between 30 - 80 micro/ml inhibited the transformation to about 50§0. Toxic effect at high doses of protamine sulphate on the bacterial cells was observed