Gas liquid chromatography as a method of assessing anaerobic bacteria in nonspecific vaginitis

ABSTRACT

Gas-liquid chromatography for short-chained organic acid metabolites of the microbial flora and quantitative anaerobic cultures were used to study the cause of nonspecific vaginitis. We analyzed vaginal fluid from normal ten women and from 25 women with nonspecific vaginitis. In normal vaginal fluid, lactate was the predominant acid, and the predominant organisms were lactobacillus and streptococcus species. In nonspecific vaginitis lactate was decreased, whereas succinate, acetate, butyrate and proprionate were increased. The predominant flora included Haemophilus vaginalis and Bacteroids