Spectrophotometric methods for the determination of dequalinium chloride and clidinium bromide using ion-pair complex formation with acid dyes

ABSTRACT

Dequlinium chloride is abisquaternary antiseptic, bactericidal against many gram-positive and gram negative bacteria, and effective against fungi. Clidinium bromide is a quatemary ammonium antimuscarinic with peripheral effects similar to those of atropine. It is used alone or with chlorodiazepoxide in pharmaceutical formulations for the symptomatic treatment of pepticulcer disease and other gastrointestinal disorders [1]. Spectrophotometric methods[2-11] have been reported for estimation of the two drugs in pure forms and in biological fluids. This paper describes the applicability of ion pair complexation reactions for spectra determination of [Deq] and [Clid] in pharmaceutical forms. The method involves the formation of ion-pair complexes between dequalinium chloride [Deq] and clidinium bromide [Clid] with BPB, BCG and BTB reagents. This method is applied successfully for the determination of [Deq] and [Clid] either in pure and in dosage forms, with good accuracy and precision. The results were compared with those given by the official method [12] where 1.2 gm of [Clid], accurately weighed, in 80 ml glacial acetic acid was added to 15 ml mercuric acetate and titrated with 0.1 M perchloric acid in dioxane. Each 1.0 ml of 0.1 M perchloric acid is equivalent to 43.24 mg of drug