Enhanced sensitivity of dor-immunobinding assays for the detection of antibodies in mice by the use of mild detergent extracted Trypanosoma Cruzi antigens

RESUMO

A method for a rapid and accurate demonstrations of antibodies to Trypanosoma Cruzy by using nitrocellulose paper strips with antigens of the parasite was developed and evaluated. We report herein additional advantages of its use for the detection of specific antibody to Trypanosoma Cruzi in the sera of mice. Increased sensibility without increase in the background was achieved using parasite antigens obtained by mild non-ionic detergent extraction as compared to antigens extracted by alkaline digestion.