An alternative method for in vitro production of HIV-1-specific antibodies
Braz. j. med. biol. res
;
24(8): 797-9, 1991. tab
Article
Dans Anglais
| LILACS
| ID: lil-102066
RESUMO
We describe the detection of HIV-1-specific antibody secretion by a 24-h culture of peripheral blood mononuclear cells (PBMC) stimulated with disrupted inactivated whole virus absorved onto commercial ELISA microwell plates. The method showed high sensitivity and specificity and was capable of accurately detecting HIV-1 infection early after birth (9 of the 19 patients were < 3 years old) because maternal antibodies do not iterfere by giving false-postive results. No false-positive results were obtained with PBMC from 24 HIV-1-seronegative asymptomatic individuals and no false-negative results were obteined for 10 seropostive adult patients (16-46 years pf age). This rapid, relatively low cost, simple, highly sensitive and specific assay can be extremely useful for the early diagnosis of pediatric AIDS cases
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Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
Agranulocytes
/
Anticorps anti-VIH
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
/
Séropositivité VIH
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Enfant d'âge préscolaire
/
Humains
/
Bébé
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1991
Type:
Article
/
Congrès et conférence
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