Selection of lacZ operon fusions in genes of gluconate metabolism in E. coli: Characterization of A gntT::lacZ fusion / Selección de fusiones lacZ de operon en genes del metabolismo del gluconato en E.coli: Caracterización de una fusión gntT::lacZ
Acta cient. venez
;
42(5): 270-5, 1991. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-105388
ABSTRACT
The initial steps involved in the utilization of gluconate by E.coli, its incorporation into the cell and susequent phosphorylation to gluconate 6-phosphate, conform two system that duplicate activities. These system, GntI and GntII, are specified by two sets of genes distinctly regulated and located respectively at the malA-asd (75 min) and fdp-valS (96 min) regions of the bacterial chromosome. The precence of duplicate activities in the metabolism of gluconates of E.coli, has made difficult the study of the expression and participation of the GntI and GntII system. In order to advance in these respec, the phage *placMu53 was used to select operon gntlacZ fusion in a E.coli strain (edd-zwf), (gnd-his), lac. Here we report the study of a gntTlacZ fusion. This transductan allowed to differentiate the inducible expresion of the gntT and gntU genes. its characteristic, in agreement with previous report, support the central role of the gntT gene in this metabolism
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Indice:
LILAS (Amériques)
Sujet Principal:
Marqueurs génétiques
/
Escherichia coli
/
Gènes bactériens
/
Gluconates
/
Opéron lac
langue:
Anglais
Texte intégral:
Acta cient. venez
Thème du journal:
Science
Année:
1991
Type:
Article
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