Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
Mem. Inst. Oswaldo Cruz
;
87(2): 239-47, abr.-jun. 1992. tab, ilus
Article
Dans Anglais
| LILACS
| ID: lil-116312
RESUMO
The present report describes an alternative method for in vitro detection of HIV-1 -specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analized. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection
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Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
/
Séropositivité VIH
/
Anticorps antiviraux
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1992
Type:
Article
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