Standardization of the dot enzyme-lynked immunosorbent assay (dot-ELISA) for experimental plague
Mem. Inst. Oswaldo Cruz
;
88(1): 119-23, jan.-mar. 1993. tab, ilus
Article
Dans Anglais
| LILACS
| ID: lil-117659
RESUMO
A dot enzyme linked immunosorbent assay (dot-ELISA) was previously developed to detect specific antibodies in rabbits sera immunized against FIA protein obtained from Yersina pestis. This antigen was covalently linked onto the surface of dacron (polyethyleneterephthalate). Here, standard conditions are described for the optimization of this procedure an amount of 20 ng of FIA protein was fixed onto dacron; anti-rabbit IgG peroxidase conjugate diluted 18,000 and 30% non-fat instant milk as blocking substance were used throughout the method. This procedure was compared with that employing nitrocellulose as solid-phase which showed to be more sensitive. However, the method based on dacron did not show false positive reactions against non-immunized rabbits sera at low antigen amount and diluted anti-IgG peroxidase conjugate
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Yersinia pestis
/
Téréphtalate polyéthylène
/
Tests enzymatiques en clinique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1993
Type:
Article
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