Expression of the VP3-VP1 sequence of foot-and-mouth disease virus in Escherichia coli
Braz. j. med. biol. res
;
26(6): 591-603, Jun. 1993. ilus
Article
Dans Anglais
| LILACS
| ID: lil-148711
ABSTRACT
1. cDNA recombinants containing the VP3 and VP1 sequences of foot-and-mouth disease virus were isolated and the VP3-VP1 sequence was reconstructed. 2. The reconstructed VP3-VP1 sequence was subcloned into expression vector pEX31b and a fusion protein of about 62,000 Da was expressed. 3. When injected into mice, the fusion protein was able to elicit the production of antibodies that recognized viral VP1 and VP3. 4. Antibodies present in sera from mice immunized with VP3-VP1 protein did not neutralize the foot-and-mouth disease virus in vitro
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Indice:
LILAS (Amériques)
Sujet Principal:
Protéines de fusion virale
/
Aphthovirus
/
Escherichia coli
/
Anticorps antiviraux
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1993
Type:
Article
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