Expression of Mycobacterium leprae 18-kDa antigen in yeast in a GPI-anchored form
Braz. j. med. biol. res
;
27(3): 623-6, Mar. 1994. ilus
Article
Dans Anglais
| LILACS
| ID: lil-148934
RESUMO
The 18-kDa protein from Mycobacterium leprae is a major target for the immune response in leprosy. We have developed a system to express this antigen in yeast as a fusion protein with the C-terminal region of the yeast membrane protein GAS1, which would render the recombinant protein anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Cells lacking the GAS1 gene and transformed with the hybrid 18-kDa-GAS1 construct express a polypeptide that reacts with an 18-kDa-specific monoclonal antibody. In addition, these cells react with an alpha-CRD antibody after GPI-PLC treatment. The non-transformed cells are negative. These data indicate that our system may be suitable for the expression of foreign proteins in yeast in a GPI-anchored form
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Indice:
LILAS (Amériques)
Sujet Principal:
Protéines bactériennes
/
Glycosylphosphatidylinositols
/
Mycobacterium leprae
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1994
Type:
Article
/
Congrès et conférence
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