Comparison of five methods for the detection of antiribosomal P protein antibody
Braz. j. med. biol. res
;
27(3): 637-43, Mar. 1994. tab
Article
Dans Anglais
| LILACS
| ID: lil-148936
RESUMO
1. We have compared the sensitivity and specificity of immunofluorescence, counterimmunoelectrophoresis, immunodiffusion, Western blotting and ELISA for the detection of antiribosomal P protein antibodies using 153 lupus sera. 2. Western blotting and ELISA were the 2 most sensitive and specific techniques for the detection of these antibodies. In contrast, cytoplasmic immunofluorescence was observed in only one third of the anti-P-positive patients. Immunodiffusion and counterimmunoelectrophoresis, although highly specific, detecting 14 per cent and 29 per cent of all anti-P-positive sera by Western blotting, were the least sensitive tests. 3. The frequency of anti-P in lupus patients, as detected by Western blotting analysis was 18 per cent . The most frequently observed antibody in anti-P sera was anti-Ro/SSA (39 per cent ). Anti-P antibodies were also detected in the sera of 3 patients with negative nuclear immunofluorescence. 4. Anti-P is an additional serological marker for systemic lupus erythematosus and Western blotting is the method of choice for detecting this antibody due to the limited availability of the fusion protein in Brazil
Recherche sur Google
Indice:
LILAS (Amériques)
Sujet Principal:
Protéines ribosomiques
/
Autoanticorps
/
Lupus érythémateux disséminé
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1994
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS