Effect of dimethyl sulfoxide on reverse transcriptase activity
Braz. j. med. biol. res
; 28(3): 285-90, Mar. 1995. tab, graf
Article
Dans En
| LILACS
| ID: lil-154692
Responsable en Bibliothèque :
BR1.1
RESUMO
Atlow concentrations, dimethyl sulfoxide (DMSO), stimulated the avian myeloblastosis virus reverse transcriptase activity. About 40 percent stimulation was obtained in the presence of 5 percent (v/v) DMSO, using activated DNA and polyriboadenylic acid (poly(rA)) as templates, and Mg2+ as divalent cation. A similar stimulation by DMSO was observed with Mn2+ for the poly(rA)-dependent reverse transcriptase activity. DMSO at concentrations higher than 15 percent inhibited the reverse transcriptase reactions, independent of the template-primers used. An exception was detected with the 2'-fluoro analog of poly(rA) as template, where an activation of 100 percent was found in the presence of 20 percent DMSO. The stimulation caused by DMSO could be due to a reduction of the apparent Km value for poly(rA) from 9.1 to 3.3 µg/ml
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Indice:
LILACS
Sujet Principal:
Diméthylsulfoxyde
/
RNA-directed DNA polymerase
/
Virus de la myéloblastose aviaire
Limites du sujet:
Animals
langue:
En
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
BIOLOGIA
/
MEDICINA
Année:
1995
Type:
Article