An enzymeimmunoassay for the determination of thyrotropin (TSH) in drieb blood on filter paper: application to a screening program for detection of congenital hypothyroidism
Rev. Hosp. Säo Paulo Esc. Paul. Med
;
5(3/4): 23-7, July-Dec. 1994. graf
Article
Dans Anglais
| LILACS
| ID: lil-155147
ABSTRACT
An enzymeimmunoassay (EIA) for H-TSH (human thyrotropin) in dried blood on filter paper using an anti-H-TSH conjugate with ß-D-galactosidase and tubes coated with an anti-H-TSH was performed fo the screening program for detection of congenital hypothyroidism. The blood volume needed in this assay was 8.7 µl. The precision was evaluated by coefficients of variance within and between assays 11.86 percent and 14.36 percent for H-TSH levels of 18.5 µU/ml and 35 µU/ml. A good correlation was observed between H-TSH concentration measured by EIA and RIA (r=0.91)
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Indice:
LILAS (Amériques)
Sujet Principal:
Thyréostimuline
/
Techniques immunoenzymatiques
/
Hypothyroïdie
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Rev. Hosp. Säo Paulo Esc. Paul. Med
Thème du journal:
Médicament
Année:
1994
Type:
Article
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