Effect of magnesium chloride and guanidinum chloride on the extraction of components of extracellular matrix from chicken cartilage
Mem. Inst. Oswaldo Cruz
;
89(1): 93-7, jan.-mar. 1994. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-155814
ABSTRACT
In order to evaluate the effect of chaotropic agents on proteoglycan and non-collagenous proteins, chicken xiphoid cartilage was treated with guanidine-HCI and MgCl2 in different concentrations (1M to 5M), and different periods of time (12, 24, 48 and 72hr). The maximum yield of uronic acid was obtained with 3M MgCl2 (73.3 per cent). Concentrations of 4M and 5M of MgCl2 showed that much less uronic acid was removed, 55.3 per cent and 38.1 respectively. Extraction with 3M MgCl2 and 3M guanidine-HCl resulted better efficiency when performed for 48 hr. Analysis by SDS-PAGE of the extracts obtained with guanidine-HCl and MgCl, in different concentrations pointed out that most components are equally removed with the two solvents, showing that the extraction with MgCl2 is an alternative assay to remove non-collagenous proteins from extracellular matrix
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cartilage
/
Chlorure de magnésium
/
Protéines de la matrice extracellulaire
/
Guanidines
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1994
Type:
Article
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