Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
Braz. j. med. biol. res
; 29(4): 489-94, Apr. 1996. graf
Article
Dans En
| LILACS
| ID: lil-163891
Responsable en Bibliothèque :
BR1.1
RESUMO
Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CLPC. The sera dilution used here was 17500. IgG (5-20 mug/ml) from the same sera, not containing beta2GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.
Recherche sur Google
Indice:
LILACS
Sujet Principal:
Cardiolipides
/
Syphilis
/
Anticorps anticardiolipines
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
langue:
En
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
BIOLOGIA
/
MEDICINA
Année:
1996
Type:
Article