Complete immunization against Trypanosoma cruzi verified in individuals mice by complement-mediated lysis
Mem. Inst. Oswaldo Cruz
;
91(1): 55-61, Jan.-Feb. 1996. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-164135
ABSTRACT
Experimental systems to assay immunity against Trypanosoma cruzi usually demonstrate partial resistance without excluding the establishment of sub-patent infections in protected animals. To test whether Swiss mice immunized with attenuated parasites might develop complete resistance against virulent T. cruzi, experiments were perfomed involving challenge with low numbers of parasites, enhancement of local inflammation and the combination of natural and acquired resistance. Absence of infection was established after repeated negative parasitological tests (including xenodiagnosis and hemoculture), and lack of lytic antibody was tested by complement mediated lysis. Immunization with 10 (7) attenuated epimastigotes conferred protection against the development of high levels of parasitemia after challenge with Tulahuen strain, but was unable to reduce the number of infected animals. However, when a strong, delayed-type hypersensitivy reaction was triggered at the site of infection by injecting a mixture of virulent and attenuated T. cruzi, a significant proportion of immunized animals remained totally free of virulent infection. The same result was obtained when the immunization experiment was perfomed in four month old Swiss mice, displaying a relatively high natural resistance and challenged with wild, vector-borne parasites. These experiments demonstrate that complete resistance against T. cruzi can be obtained in a significant proportion of animals, under conditions which replicate natural, vector delivered infection by the parasite.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Résistance microbienne aux médicaments
/
Immunisation
/
Souris
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1996
Type:
Article
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