Diagnosis of malaria by acridine orange fluorescent microscopy in an endemic area of Venezuela
Mem. Inst. Oswaldo Cruz
;
91(1): 83-6, Jan.-Feb. 1996. tab
Article
Dans Anglais
| LILACS
| ID: lil-164140
ABSTRACT
Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC] analysis) and Giemsa stained thick blood smears (GTS) were compared for diagnosis of malaria in blood specimens from adults living in malaria transmission areas of the states of Bolivar and Amazonas in southeastern and south Venezuela, respectively. Of a total of 198 GTS examined, 95 subjects (48 por cento) showed parasitaemia. Among the 95 blood films with a positive GTS, 94 were judged positive by the QBC. However, positive QBC tubes were found in 29 out of 103 blood specimens with a negative GTS. Thus, relative to a GTS standard, the sensitivity and specificity of the QBC-test was 99.2 por cento and 72 por cento, respectively. Young trophozoites of Plasmodium vivax and P. falciparum could not be distinguished with certainty. It is confirmed that the OBC offers many advantages compared with the standard diagnosis of malaria parasites, specifically in the speed of staining and ease of interpretation. However, in places where P. falciparum and P. vivax occur, species and stage differentation should be confirmed with the GTS.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Zones de pauvreté
/
Paludisme
/
Microscopie de fluorescence
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
Pays comme sujet:
Amérique du Sud
/
Vénézuela
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1996
Type:
Article
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