The use of polyvinyl alcohol glutaraldehyde as solid-phase in ELISA for plague
Mem. Inst. Oswaldo Cruz
;
91(2): 195-8, Mar.-Apr. 1996. tab
Article
Dans Anglais
| LILACS
| ID: lil-174379
RESUMO
Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3µg of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS); anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3 per cent w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 112,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Peste
/
Poly(alcool vinylique)
/
Test ELISA
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1996
Type:
Article
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