The haemoculture of Trypanosoma minasense Chagas, 1908
Mem. Inst. Oswaldo Cruz
;
91(4): 501-5, July-Aug. 1996. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-174410
ABSTRACT
Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20 per cent foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN), at 27§C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacycliclike forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma
/
Callithrix
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1996
Type:
Article
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