Hepatitis C viral RNA amplification by the polymerase chain reaction allows detection of false positive HCV ELISAs among patients with non-A, non-B hepatitis
P. R. health sci. j
; P. R. health sci. j;12(1): 35-8, abr. 1993.
Article
de En
| LILACS
| ID: lil-176721
Bibliothèque responsable:
BR1.1
RESUMO
Serum from patients which tested positive for hepatitis C virus (HCV) by Enzyme Linked Immunosorbent Assay (ELISA) were analyzed for the presence of HCV RNA by nested Polymerase Chain Reaction (PCR) and for anti-HCV antibodies by Recombinant Immunoblot Assay (RIBA II). Total RNA was extracted from whole blood by a new procedure and subjected to reverse transcription of HCV RNA employing primers to the conserved 5' non-coding region of the HCV genome. PCR performed on these samples uncovered several false positive ELISAs. Reciprocal confirmation between PCR and RIBA II results was observed. These results substantiate this variation of the HCV PCR assay as a reliable alternative for routine confirmation of HCV serological tests
Recherche sur Google
Indice:
LILACS
Sujet Principal:
ARN viral
/
Réaction de polymérisation en chaîne
/
Hépatite C
/
Hepacivirus
Type d'étude:
Diagnostic_studies
Limites du sujet:
Adult
/
Female
/
Humans
/
Male
langue:
En
Texte intégral:
P R Health Sci J
/
P. R. health sci. j
/
Puerto Rico health sciences journal
/
Puerto Rico health sciences journal (Online)
Thème du journal:
MEDICINA
Année:
1993
Type:
Article